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Humanized Antibody Preparation Strategy

Tuesday , 23, March 2021 Comments Off on Humanized Antibody Preparation Strategy

The humanized antibody mainly refers to that, which is re-expressed by the mouse monoclonal antibody by gene cloning and DNA recombination technology, and most of the amino acid sequence is replaced by the human sequence and specificity of the parent monoclonal mouse is maintained substantially and the antibody is reduced.

Its heterogeneity benefits the human body. If you want to know more about humanized antibody preparation, then you can also check out the Boster Bio featured products.

The variable region of the murine antibody gene was recombined with the constant region of the human antibody gene by recombinant DNA technology, and the recombinant gene was inserted into myeloma cells for expression.

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Depending on the plasmid vector used to label the gene product, the antibiotic or suitable preparation used for screening and the chimeric secreting cell line in human mice was cloned in a manner similar to conventional techniques.

FR in region V from mouse antibodies still retains some immunogenicity. These are very far from truly humanized antibodies, and some can produce a strong anti-idiotypic response.

To reduce the composition of mice, humans attempted to replace mouse FR with human FR to produce a more complete range of antibodies from the humanization antibody service, with the exception that all three CDRs were mouse sources, all of which were human structures, also known as CDR-grafted antibodies.

The manufacture of phage antibody library technology relies on the development of three experimental techniques: One is the development of PCR technology, which allows one to clone a complete set of immunoglobulin variable regions of total B lymphocyte RNA by RP-TCR.

Making antibody library construction simple and easy to manipulate. One of these is the development of phage display technology to unify genotype and phenotype, which provides a highly efficient screening system that supports phage antibody technology.